KMID : 1007520190280041171
|
|
Food Science and Biotechnology 2019 Volume.28 No. 4 p.1171 ~ p.1176
|
|
Improved astaxanthin production by Xanthophyllomyces dendrorhous SK984 with oak leaf extract and inorganic phosphate supplementation
|
|
Kothari Damini
Lee Jun-Hyeong Chon Jung-Whan Seo Kun-Ho Kim Soo-Ki
|
|
Abstract
|
|
|
Astaxanthin is widely used in food, feed and nutraceutical industries. Xanthophyllomyces dendrorhous is one of the most promising natural sources of astaxanthin. However, the astaxanthin yield in the wild-type X. dendrorhous is considered low for industrial application. In the present study, X. dendrorhous ATCC 66272 was subjected to two-staged mutagenesis: (i) UV light and (ii) N-methyl-N¡Ç-nitro-N-nitroso-guanidine (NTG) toward attaining higher astaxanthin yield. The UV-irradiation mutant, X. dendrorhous SK974 showed 1.7-fold (1.07 mg/g) higher astaxanthin production as compared with the wild-type strain (0.65 mg/g). The UV mutant strain was then treated with NTG, designated as X. dendrorhous SK984, displayed further 1.4-fold (1.45 mg/g) higher astaxanthin production. Furthermore, the oak leaf extract (5%, v/v) and inorganic phosphate (KH2PO4, 3 mM) supplementation resulted about 1.4-fold (1.98 mg/g) higher astaxanthin production as compared with control (1.45 mg/g) in X. dendrorhous SK984. These findings serve as a platform suggesting that intersecting approaches might be aimed toward systematically enhanced astaxanthin production.
|
|
KEYWORD
|
|
Astaxanthin, Inorganic phosphate, Mutagenesis, Oak leaf extract, X. dendrorhous
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|